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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Collagen Type XI Polyclonal antibody (21841-1-AP)

    WB analysis using 21841-1-AP (same clone as 21841-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Collagen Type XI Polyclonal antibody (21841-1-AP)

    IHC staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Collagen Type XI Polyclonal antibody (21841-1-AP)

    IHC staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Collagen Type XI Polyclonal antibody (21841-1-AP)

    IHC staining of human stomach cancer using 21841-1-AP (same clone as 21841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Collagen Type XI Polyclonal antibody (21841-1-AP)

    IHC staining of human stomach cancer using 21841-1-AP (same clone as 21841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Collagen Type XI Polyclonal antibody (21841-1-AP)

    IF Staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Collagen Type XI antibody (21841-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

Collagen Type XI Polyclonal antibody, PBS Only

Collagen Type XI Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 21841-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

COL11, COL11A1, CO11A1, COLL6, Collagen alpha 1(XI) chain

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21841-1-PBS targets Collagen Type XI in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16509

Product name: Recombinant human COL11A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1429-1690 aa of BC117697

Sequence: PLPILSSKKTRRHTEGMQADADDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTSGGETCIYPDKKSEGVRISSWPKEKPGSWFSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYHCHQSAAWYDVSSGSYDKALRFLGSNDEEMSYDNNPFIKTLYDGCASRKGYEKTVIEINTPKIDQVPIVDVMINDFGDQNQKFGFEVGPVCFLG

相同性解析による交差性が予測される生物種
Full Name collagen, type XI, alpha 1
Calculated molecular weight 1818 aa, 182 kDa
Observed molecular weight250 kDa, 181 kDa
GenBank accession numberBC117697
Gene Symbol COL11A1
Gene ID (NCBI) 1301
RRIDAB_2918074
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP12107
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

COL11A1 is an important component of collagen XI and is considered to play an important role in a variety of connective tissue diseases. Type XI collagen is widely distributed in tissues including cartilage, bone, and muscle, and its hereditary absence in Marshall's syndrome, Stickler's syndrome, fibrochondrogenesis, and nonsyndromic hearing loss deafness, autosomal dominant 37 leads to facial and eye abnormalities, hearing loss, and joint problems. The COL11A1 expression was significantly correlated with the degree of immune infiltration and the expression of a variety of immune cell markers in tumor tissue. (PMID: 36160004 33610546)

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