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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Collagen Type IV Polyclonal antibody (30850-1-AP)

    WB analysis using 30850-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30850-1-AP (COL4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Collagen Type IV Polyclonal antibody (30850-1-AP)

    IHC staining of human lung cancer using 30850-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30850-1-AP (Collagen Type IV antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Collagen Type IV Polyclonal antibody (30850-1-AP)

    IHC staining of human placenta using 30850-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30850-1-AP (Collagen Type IV antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Collagen Type IV Polyclonal antibody (30850-1-AP)

    IHC staining of human placenta using 30850-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30850-1-AP (Collagen Type IV antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Collagen Type IV Polyclonal antibody (30850-1-AP)

    IHC staining of human lung cancer using 30850-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30850-1-AP (Collagen Type IV antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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COL4A1 Polyclonal antibody

COL4A1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30850-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

arresten, Collagen alpha 1(IV) chain, Collagen alpha-1(IV) chain, Collagen Type IV

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, SK-N-SH cells
Positive IHC detected inhuman lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

30850-1-AP targets COL4A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COL4A1 fusion protein Ag34391 相同性解析による交差性が予測される生物種
Full Name collagen, type IV, alpha 1
Calculated molecular weight 1669 aa, 161 kDa
Observed molecular weight160-220 kDa
GenBank accession numberBC151220
Gene Symbol COL4A1
Gene ID (NCBI) 1282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP02462
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COL4A1 (Collagen Type IV Alpha 1 Chain) encodes a major component of type IV collagen, which is an essential structural element of basement membranes in various tissues. COL4A1 forms heterotrimers with other collagen type IV chains (e.g., COL4A2) and interacts with extracellular matrix components such as laminins, proteoglycans, and perlecans. Proteolytic cleavage of the non-collagenous domain of COL4A1 produces a fragment called arresten, which has anti-angiogenic and tumor suppressor properties. Elevated expression of COL4A1 has been observed in various cancers, including hepatocellular carcinoma (HCC), where it promotes tumor growth and metastasis through the FAK-Src signaling pathway. COL4A1 is highly expressed in various tissues, including the placenta, fat, and other organs. The expression of COL4A1 can be regulated by transcription factors such as RUNX1, which has been shown to upregulate COL4A1 in hepatocellular carcinoma. The molecular weight of OSBPL5 is 160 kDa, and it also has glycosylation modification.

Protocols

Product Specific Protocols
WB protocol for COL4A1 antibody 30850-1-APDownload protocol
IHC protocol for COL4A1 antibody 30850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Sci Rep

Yiqi Juanshen decoction alleviates renal interstitial fibrosis by targeting the LOXL2/PI3K/AKT pathway to suppress EMT and inflammation

Authors - Kaiyue Tan
View Article