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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cofilin Recombinant monoclonal antibody (88053-1-RR)

    WB analysis using 88053-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88053-1-RR (Cofilin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human appendix tissue using Cofilin Recombinant monoclonal antibody (88053-1-RR)

    IHC staining of human appendix using 88053-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 88053-1-RR (Cofilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cofilin Recombinant monoclonal antibody

Cofilin Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 88053-1-RR
Clone No.260317G7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CFL1, 18 kDa phosphoprotein, Cofilin 1, Cofilin, non-muscle isoform, Cofilin1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HeLa cells, LNCaP cells, Jurkat cells, HEK-293 cells, Neuro-2a cells, PC-12 cells
Positive IHC detected inhuman appendix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88053-1-RR targets Cofilin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1399

Product name: Recombinant human Cofilin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-166 aa of BC012318

Sequence: MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL

相同性解析による交差性が予測される生物種
Full Name cofilin 1 (non-muscle)
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC012318
Gene Symbol Cofilin
Gene ID (NCBI) 1072
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP23528
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Protocols

Product Specific Protocols
IHC protocol for Cofilin antibody 88053-1-RRDownload protocol
WB protocol for Cofilin antibody 88053-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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