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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Coagulation Factor II Polyclonal antibody (31615-1-AP)

    WB analysis using 31615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Coagulation Factor II Polyclonal antibody (31615-1-AP)

    WB analysis using 31615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Coagulation Factor II Polyclonal antibody (31615-1-AP)

    IHC staining of mouse liver using 31615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Coagulation Factor II Polyclonal antibody (31615-1-AP)

    IHC staining of mouse liver using 31615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Coagulation Factor II Polyclonal antibody (31615-1-AP)

    IF Staining of HepG2 using 31615-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Coagulation Factor II antibody (31615-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Coagulation Factor II Polyclonal antibody

Coagulation Factor II Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31615-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

F2, Prothrombin, Activation peptide fragment 1, Activation peptide fragment 2, PT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma, mouse heart tissue, rat plasma, mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31615-1-AP targets Coagulation Factor II in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name coagulation factor II
Calculated molecular weight70 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberNM_010168.3
Gene Symbol F2
Gene ID (NCBI) 14061
RRIDAB_3670049
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19221
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin or factor II, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis. This antibody specifically recognizes mouse Coagulation Factor II.

Protocols

Product Specific Protocols
WB protocol for Coagulation Factor II antibody 31615-1-APDownload protocol
IHC protocol for Coagulation Factor II antibody 31615-1-APDownload protocol
IF protocol for Coagulation Factor II antibody 31615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols