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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse serum using Clusterin Polyclonal antibody (30339-1-AP)

    WB analysis of mouse serum using 30339-1-AP

    mouse serum were subjected to SDS PAGE followed by western blot with 30339-1-AP (Clusterin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Clusterin Polyclonal antibody (30339-1-AP)

    WB analysis using 30339-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30339-1-AP (Clusterin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Clusterin Polyclonal antibody (30339-1-AP)

    IHC staining of human liver using 30339-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30339-1-AP (Clusterin antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Clusterin Polyclonal antibody (30339-1-AP)

    IHC staining of human liver using 30339-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30339-1-AP (Clusterin antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Clusterin Polyclonal antibody (30339-1-AP)

    IHC staining of human prostate cancer using 30339-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30339-1-AP (Clusterin antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Clusterin Polyclonal antibody (30339-1-AP)

    IHC staining of human prostate cancer using 30339-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30339-1-AP (Clusterin antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Clusterin Polyclonal antibody (30339-1-AP)

    IF Staining of HeLa using 30339-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Clusterin antibody (30339-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Clusterin Polyclonal antibody

Clusterin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30339-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AAG4, Aging-associated gene 4 protein, Apo J, APOJ, Apo-J

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma, mouse serum
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30339-1-AP targets Clusterin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Eg0225

Product name: Recombinant Human CLU protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 23-449 aa of BC010514

Sequence: DQTVSDNELQEMSNQGSKYVNKEIQNAVNGVKQIKTLIEKTNEERKTLLSNLEEAKKKKEDALNETRESETKLKELPGVCNETMMALWEECKPCLKQTCMKFYARVCRSGSGLVGRQLEEFLNQSSPFYFWMNGDRIDSLLENDRQQTHMLDVMQDHFSRASSIIDELFQDRFFTREPQDTYHYLPFSLPHRRPHFFFPKSRIVRSLMPFSPYEPLNFHAMFQPFLEMIHEAQQAMDIHFHSPAFQHPPTEFIREGDDDRTVCREIRHNSTGCLRMKDQCDKCREILSVDCSTNNPSQAKLRRELDESLQVAERLTRKYNELLKSYQWKMLNTSSLLEQLNEQFNWVSRLANLTQGEDQYYLRVTTVASHTSDSDVPSGVTEVVVKLFDSDPITVTVPVEVSRKNPKFMETVAEKALQEYRKKHREE

相同性解析による交差性が予測される生物種
Full Name clusterin
Calculated molecular weight 449 aa, 53 kDa
Observed molecular weight34-39 kDa
GenBank accession numberBC010514
Gene Symbol CLU
Gene ID (NCBI) 1191
RRIDAB_3669713
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP10909
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

Protocols

Product Specific Protocols
IF protocol for Clusterin antibody 30339-1-APDownload protocol
IHC protocol for Clusterin antibody 30339-1-APDownload protocol
WB protocol for Clusterin antibody 30339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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