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Cleaved PARP1 (Asp214) Recombinant antibody

Cleaved PARP1 (Asp214) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85175-5-RR
Clone No.243142B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

PARP1, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, ADPRT 1, ADPRT1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inStaurosporine treated HeLa cells, Staurosporine treated Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85175-5-RR targets Cleaved PARP1 (Asp214) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase 1
Calculated molecular weight 1014 aa, 113 kDa
Observed molecular weight89 kDa
GenBank accession numberBC037545
Gene Symbol PARP1
Gene ID (NCBI) 142
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24 kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89 kDa.
This antibody only recognizes the cleaved form of PAPR1 but not full-length PARP1.

Protocols

Product Specific Protocols
WB protocol for Cleaved PARP1 (Asp214) antibody 85175-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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