Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, MDA-MB-231 cells, human placenta tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86433-8-RR targets Claudin 4 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | claudin 4 |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 18-20 kDa |
| GenBank accession number | BC000671 |
| Gene Symbol | Claudin 4 |
| Gene ID (NCBI) | 1364 |
| RRID | AB_3744879 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O14493 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Claudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They are small (20-27 kDa) proteins with similar structures. They have four transmembrane domains, with the N- and C-termini in the cytoplasm. This antibody is specifically against Claudin 4. Claudin 4 is overexpressed in a variety of cancers. It can be used as a predictor of recurrent malignant pleural effusion in patients with advanced non-small cell lung cancer (PMID: 38629624).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Claudin 4 antibody 86433-8-RR | Download protocol |
| WB protocol for Claudin 4 antibody 86433-8-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |