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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Chromogranin B Polyclonal antibody

Chromogranin B Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 28663-1-AP

Synonyms

CgB, CHGB, Chromogranin B, SCG1, Secretogranin 1, Secretogranin I, SgI

Validation Data Gallery

Applications:
  • WB analysis using 28663-1-AP

    WB analysis using 28663-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 28663-1-AP (Chromogranin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of PC-12 using 28663-1-AP

    FC experiment of PC-12 using 28663-1-AP

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Chromogranin B (28663-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inPC-12 cells
Positive FC detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy CHGB Ready-To-Use IHC Kit. CHGB primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

28663-1-AP targets Chromogranin B in WB, FC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chromogranin B fusion protein Ag29920 相同性解析による交差性が予測される生物種
Full Name chromogranin B (secretogranin 1)
Calculated molecular weight 78 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC000375
Gene symbol CHGB
Gene ID (NCBI) 1114
RRIDAB_2881188
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Chromogranin B antibody 28663-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols