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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Chk1 Monoclonal antibody (60277-1-Ig)

    WB analysis using 60277-1-Ig (same clone as 60277-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60277-1-Ig (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Chk1 Monoclonal antibody (60277-1-Ig)

    WB analysis of NIH/3T3 using 60277-1-Ig (same clone as 60277-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60277-1-Ig (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Chk1 Monoclonal antibody (60277-1-Ig)

    IHC staining of human breast cancer using 60277-1-Ig (same clone as 60277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60277-1-Ig (Chk1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Chk1 Monoclonal antibody (60277-1-Ig)

    IHC staining of human breast cancer using 60277-1-Ig (same clone as 60277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60277-1-Ig (Chk1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

Chk1 Monoclonal antibody, PBS Only

Chk1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60277-1-PBS
Clone No.3A3A5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CHEK1, Chk1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60277-1-PBS targets Chk1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0409

Product name: Recombinant human CHK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 42-256 aa of BC004202

Sequence: MKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITI

相同性解析による交差性が予測される生物種
Full Name CHK1 checkpoint homolog (S. pombe)
Calculated molecular weight 54 kDa
Observed molecular weight50 kDa
GenBank accession numberBC004202
Gene Symbol Chk1
Gene ID (NCBI) 1111
RRIDAB_2881396
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO14757
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.