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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Chemerin Recombinant monoclonal antibody (83550-4-RR)

    WB analysis using 83550-4-RR (same clone as 83550-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83550-4-RR (Chemerin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83550-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of C57BL/6 mouse liver cells using Chemerin Recombinant monoclonal antibody (83550-4-RR)

    FC experiment of C57BL/6 mouse liver cells using 83550-4-RR (same clone as 83550-4-PBS)

    1x10^6 C57BL/6 mouse liver cells were intracellularly stained with 0.25 ug Chemerin Recombinant antibody (83550-4-RR, Clone:240355A12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83550-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83550-4-RR

    Affinity and Kinetic Characterization of 83550-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83550-4-RR against Mouse Chemerin were performed. The affinity constant is 3.76 nM.

Mouse Chemerin Recombinant monoclonal antibody, PBS Only

Chemerin Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83550-4-PBS
Clone No.240355A12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, FC (Intra), ELISA

Synonyms

Rarres2, 240355A12, AI303516, Retinoic acid receptor responder protein 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83550-4-PBS targets Chemerin in WB, FC (Intra), ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0875

Product name: Recombinant mouse Chemerin protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 21-156 aa of NM_001347168.1

Sequence: EPELSETQRRSLQVALEEFHKHPPVQLAFQEIGVDRAEEVLFSAGTFVRLEFKLQQTNCPKKDWKKPECTIKPNGRRRKCLACIKMDPKGKILGRIVHCPILKQGPQDPQELQCIKIAQAGEDPHGYFLPGQFAFS

相同性解析による交差性が予測される生物種
Full Name retinoic acid receptor responder (tazarotene induced) 2
Calculated molecular weight18 kDa
GenBank accession numberNM_001347168.1
Gene Symbol Chemerin
Gene ID (NCBI) 71660
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9DD06
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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