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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Centrin 2 Recombinant monoclonal antibody (88406-1-RR)

    WB analysis using 88406-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88406-1-RR (Centrin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Centrin 2 Recombinant monoclonal antibody (88406-1-RR)

    WB analysis using 88406-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88406-1-RR (Centrin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Centrin 2 Recombinant monoclonal antibody (88406-1-RR)

    WB analysis of mouse brain using 88406-1-RR

    Mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 88406-1-RR (Centrin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Centrin 2 Recombinant monoclonal antibody

Centrin 2 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 88406-1-RR
Clone No.260861B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CETN2, CALT, Caltractin isoform 1, CEN2, Centrin-2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue, HT-29 cells, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88406-1-RR targets Centrin 2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8638

Product name: Recombinant human Centrin 2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-172 aa of BC005334

Sequence: MASNFKKANMASSSQRKRMSPKPELTEEQKQEIREAFDLFDADGTGTIDVKELKVAMRALGFEPKKEEIKKMISEIDKEGTGKMNFGDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVSEQEFLRIMKKTSLY

相同性解析による交差性が予測される生物種
Full Name centrin, EF-hand protein, 2
Calculated molecular weight 172 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC005334
Gene Symbol Centrin 2
Gene ID (NCBI) 1069
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP41208
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Centrin 2 is also named as CALT, or CEN2. It plays a fundamental role in microtubule-organizing center structure and function. Oxidizing radiation induces polymerization of centrin 2 into dimers, tetramers, hexamers,and even higher molecular mass species, as a function of the radiation dose(PMID:17603931).It is specifically localized to the centrosome of interphase and mitotic cells in both HeLa and BHK cells (PMID:8248209).

Protocols

Product Specific Protocols
WB protocol for Centrin 2 antibody 88406-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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