Validation Data Gallery

  • Live cell imaging of HeLa cells: Live cell imaging of HeLa cells stable expressing Cell Cycle-Chromobody (TagRFP). Images from a time series of 22 hours are shown

    Live cell imaging of HeLa cells: Live cell imaging of HeLa cells stable expressing Cell Cycle-Chromobody (TagRFP). Images from a time series of 22 hours are shown

  • Cell Cycle-Chromobody signal during the cell cycle: In G1, the Chromobody signal is homogeneously distributed throughout the nucleus and cytoplasm. During S phase it accumulates in the nucleus and visualizes the formation of replication foci. In G2, the foci disappear and the cell divides (mitosis).

    Cell Cycle-Chromobody signal during the cell cycle: In G1, the Chromobody signal is homogeneously distributed throughout the nucleus and cytoplasm. During S phase it accumulates in the nucleus and visualizes the formation of replication foci. In G2, the foci disappear and the cell divides (mitosis).

  • Time lapse video: Live cell imaging of the stable cell line HeLa-Cell Cycle Chromobody TagRFP expressing Cell Cycle-Chromobody.

    Time lapse video: Live cell imaging of the stable cell line HeLa-Cell Cycle Chromobody TagRFP expressing Cell Cycle-Chromobody.

ChromoTek Cell Cycle Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker.
Cat No. ccr

Specificity

PCNA

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagRFP

Chromobody, PCNA Chromobody

Size/Concentration: 

-/ -


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Product Information

DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker.

DescriptionThe Cell Cycle-Chromobody allows visualization of the endogenous cell cycle marker protein PCNA:

• Trace dynamic changes during the cell cycle in real-time

• Monitor the distribution of an endogenous cell cycle marker protein without artifacts or cytotoxic effects when overexpressing fluorescent fusion proteins

• Does not affect cellular functions

ApplicationsLive cell imaging
Visualizing endogenous cell cycle marker protein PCNA
Actin as biomarker or control
SpecificityPCNA (proliferating cell nuclear antigen), tested in human cells and zebrafish
ReporterTagRFP
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SelectionKan/Neo
SequenceWith the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence.
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis
Storage ConditionShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Nature

Equilibrium between nascent and parental MCM proteins protects replicating genomes.

Authors - Hana Sedlackova

Nature

CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibition.

Authors - David Gallo

Nat Cell Biol

53BP1 nuclear bodies enforce replication timing at under-replicated DNA to limit heritable DNA damage.

Authors - Julian Spies

Nat Cell Biol

Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress.

Authors - Noa Lamm
IHC

Mol Cell

DNA Replication Determines Timing of Mitosis by Restricting CDK1 and PLK1 Activation.

Authors - Bennie Lemmens

EMBO J

Evidence for the nuclear import of histones H3.1 and H4 as monomers.

Authors - Michael James Apta-Smith