Cell Cycle Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker.

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(希望販売価格)

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カタログ番号:
ccr

Application:
Live cell imaging

Specificity:
PCNA (proliferating cell nuclear antigen), tested in human cells and zebrafish

Vector type:
Mammalian expression vector

Promoter:
Constitutive CMV IE

Reporter:
TagRFP

Codon usage:
Mammalian

Selection:
Kan/Neo

Encoded Nanobody/ VHH:
Monoclonal anti-PCNA single domain antibody (sdAb) fragment

Sequence:
With the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence.

Microscopy techniques:
Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis

Transfection:
Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.

Storage:
Shipped at ambient temperature. Store at -20 °C

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