Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-12 cells, NIH/3T3 cells, DC2.4 cells, HSC-T6 cells, RAW 264.7 cells, Sp2/0 cells, Neuro-2a cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 79 publications below |
| IHC | See 16 publications below |
| IF | See 20 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
28248-1-AP targets p21 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28394 Product name: Recombinant mouse Cdkn1a protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-159 aa of NM_001111099 Sequence: MSNPGDVRPVPHRSKVCRCLFGPVDSEQLRRDCDALMAGCLQEARERWNFDFVTETPLEGNFVWERVRSLGLPKVYLSPGSRSRDDLGGDKRPSTSSALLQGPAPEDHVALSLSCTLVSERPEDSPGGPGTSQGRKRRQTSLTDFYHSKRRLVFCKRKP 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin-dependent kinase inhibitor 1A (P21) |
| Calculated molecular weight | 18 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | NM_001111099 |
| Gene Symbol | p21 |
| Gene ID (NCBI) | 12575 |
| RRID | AB_2881097 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P39689 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for p21 antibody 28248-1-AP | Download protocol |
| IP protocol for p21 antibody 28248-1-AP | Download protocol |
| WB protocol for p21 antibody 28248-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma | ||
Redox Biol Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice | ||
EMBO J Kinesin-like motor protein KIF23 maintains neural stem and progenitor cell pools in the developing cortex | ||
Dev Cell Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity. | ||
Aging Cell Excessive processing and acetylation of OPA1 aggravate age-related hearing loss via the dysregulation of mitochondrial dynamics | ||
Biomed Pharmacother Seno-antigen-pulsed dendritic cell vaccine induce anti-aging immunity to improve adipose tissue senescence and metabolic abnormalities |
