Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of mouse kidney using 51046-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 51046-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 51046-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 51046-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 51046-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 51046-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 51046-1-AP
IP result of anti-Cd2ap (IP:51046-1-AP, 4ug; Detection:51046-1-AP 1:2000) with mouse brain tissue lysate 2560ug.
IP experiment of HeLa cells using 51046-1-AP
IP result of anti-Cd2AP (51046-1-AP for IP and Detection).
IF Staining of HepG2 using 51046-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 51046-1-AP (Cd2ap antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A431 using 51046-1-AP
Immunofluorescent analysis of A431 cells using 51046-1-AP (Cd2ap antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

CD2AP Polyclonal antibody

CD2AP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 51046-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, CoIP, ELISA

Synonyms

METS-1, Mets1, METS 1, Mesenchyme-to-epithelium transition protein with SH3 domains 1, CD2-associated protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, mouse testis tissue, mouse brain tissue, Jurkat cells, A431 cells, HeLa cells
Positive IP detected in	mouse brain tissue, HeLa cells
Positive IF/ICC detected in	HepG2 cells, A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:20000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 8 publications below
IF	See 4 publications below
CoIP	See 1 publications below

Product Information

51046-1-AP targets CD2AP in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0622 Product name: Recombinant mouse Cd2ap protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-637 aa of BC157901 Sequence: MVDYIVEYDYDAVHDDELTIRVGEIIRNVKKLQEEGWLEGELNGRRGMFPDNFVKEIKRETEPKDDNLPIKRERQGNVASLVQRISTYGLPAGGIQPHPQTKAIKKKTKKRQCKVLFDYSPQNEDELELIVGDVIDVIEEVEEGWWSGTLNNKLGLFPSNFVKELESTEDGETHNAQEESEVPLTGPTSPLPSPGNGSEPAPGSVAQPKKIRGIGFGDIFKEGSVKLRTRTSSSETEEKKTEKPLILQPLGSRTQNVEVTKPDVDGKIKAKEYCRTLFPYTGTNEDELTFREGEIIHLISKETGEAGWWKGELNGKEGVFPDNFAVQISELDKDFPKPKKPPPPAKGPAPKPDLSAAEKKAFPLKAEEKDEKSLLEQKPSKPAAPQVPPKKPTAPTKASNLLRSPGAVYPKRPEKPVPPPPPAAKINGEVSIISSKIDTEPVSKPKLDPEQLPVRPKSVDLDAFVARNSKETDDVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGGHSPTQSPEKTLKLPKEDDSGNLKPLEFKKDASYSSKPSLSTPSSASKVNTAAFLTPLELKAKAEADDGKKNSVDELRAQIIELLCIVDALKKDHGKELEKLRKELEEEKAMRSNLEVEIAKLKKAVLLS 相同性解析による交差性が予測される生物種
Full Name	CD2-associated protein
Calculated molecular weight	637 aa, 70 kDa
Observed molecular weight	71 kDa
GenBank accession number	BC157901
Gene Symbol	Cd2ap
Gene ID (NCBI)	12488
RRID	AB_670322
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9JLQ0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).

Protocols

Product Specific Protocols
IF protocol for CD2AP antibody 51046-1-AP	Download protocol
IP protocol for CD2AP antibody 51046-1-AP	Download protocol
WB protocol for CD2AP antibody 51046-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	J Clin Invest Endoplasmic reticulum-associated degradation is required for nephrin maturation and kidney glomerular filtration function. Authors - Sei Yoshida View Article
human	WB	Exp Ther Med Transforming growth factor-β1-induced podocyte injury is associated with increased microRNA-155 expression, enhanced inflammatory responses and MAPK pathway activation. Authors - Xintong Zheng View Article
human	WB,IF	J Biol Chem The Rab GTPase-binding protein EHBP1L1 and its interactors CD2AP/CIN85 negatively regulate the length of primary cilia via actin remodeling Authors - Tomohiko Iwano KD Validated View Article
mouse	WB	Int J Mol Sci The Cleavage-Specific Tau 12A12mAb Exerts an Anti-Amyloidogenic Action by Modulating the Endocytic and Bioenergetic Pathways in Alzheimer's Disease Mouse Model Authors - Valentina Latina View Article
mouse	WB,IF	J Am Soc Nephrol Glomerulosclerosis Induced by Deficiency of Membrane-Associated Guanylate Kinase Inverted 2 in Kidney Podocytes Authors - Naritoshi Shirata View Article
human	WB	Int J Cardiol Human cytomegalovirus pUL135 protein affects endothelial cell function via CD2AP in Kawasaki disease Authors - Lili Yu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse kidney using 51046-1-AP

WB analysis of Jurkat using 51046-1-AP

WB analysis of mouse brain using 51046-1-AP

WB analysis of HeLa using 51046-1-AP

WB analysis of A431 using 51046-1-AP

WB analysis of mouse testis using 51046-1-AP

IP experiment of mouse brain using 51046-1-AP

IP experiment of HeLa cells using 51046-1-AP

IF Staining of HepG2 using 51046-1-AP

IF Staining of A431 using 51046-1-AP

CD2AP Polyclonal antibody

CD2AP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Endoplasmic reticulum-associated degradation is required for nephrin maturation and kidney glomerular filtration function.

Transforming growth factor-β1-induced podocyte injury is associated with increased microRNA-155 expression, enhanced inflammatory responses and MAPK pathway activation.

The Rab GTPase-binding protein EHBP1L1 and its interactors CD2AP/CIN85 negatively regulate the length of primary cilia via actin remodeling

The Cleavage-Specific Tau 12A12mAb Exerts an Anti-Amyloidogenic Action by Modulating the Endocytic and Bioenergetic Pathways in Alzheimer's Disease Mouse Model

Glomerulosclerosis Induced by Deficiency of Membrane-Associated Guanylate Kinase Inverted 2 in Kidney Podocytes

Human cytomegalovirus pUL135 protein affects endothelial cell function via CD2AP in Kawasaki disease