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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Caveolin-1 Recombinant antibody (84044-1-RR)

    WB analysis using 84044-1-RR (same clone as 84044-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84044-1-RR (Caveolin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Caveolin-1 Recombinant antibody (84044-1-RR)

    IHC staining of human colon using 84044-1-RR (same clone as 84044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Caveolin-1 Recombinant antibody (84044-1-RR)

    IHC staining of human colon cancer using 84044-1-RR (same clone as 84044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung squamous cell carcinoma tissue using Caveolin-1 Recombinant antibody (84044-1-RR)

    IHC staining of human lung squamous cell carcinoma using 84044-1-RR (same clone as 84044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84044-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84044-1-RR

    Affinity and Kinetic Characterization of 84044-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84044-1-RR against Human Caveolin-1 were performed. The affinity constant is 1.53 nM.

Caveolin-1 Recombinant monoclonal antibody, PBS Only

Caveolin-1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84044-1-PBS
Clone No.240985E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Caveolin 1, CAV1, CGL3, CAV, BSCL3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84044-1-PBS targets Caveolin-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8049

Product name: Recombinant human Caveolin-1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 11-179 aa of BC006432

Sequence: GHLYTVPIREQGNIYKPNNKAMADELSEKQVYDAHTKEIDLVNRDPKHLNDDVVKIDFEDVIAEPEGTHSFDGIWKASFTTFTVTKYWFYRLLSALFGIPMALIWGIYFAILSFLHIWAVVPCIKSFLIEIQCISRVYSIYVHTVCDPLFEAVGKIFSNVRINLQKEI

相同性解析による交差性が予測される生物種
Full Name caveolin 1, caveolae protein, 22kDa
Calculated molecular weight 22 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC006432
Gene Symbol CAV1
Gene ID (NCBI) 857
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ03135
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).