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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cathepsin K Polyclonal antibody (11239-1-AP)

    WB analysis using 11239-1-AP (same clone as 11239-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 11239-1-AP (Cathepsin K antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of ROS1728 cells using Cathepsin K Polyclonal antibody (11239-1-AP)

    IP experiment of ROS1728 using 11239-1-AP (same clone as 11239-1-PBS)

    IP result of anti-Cathepsin K (IP:11239-1-AP, 3ug; Detection:11239-1-AP 1:500) with ROS1728 cells lysate 3200ug. This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cathepsin K Polyclonal antibody (11239-1-AP)

    IHC staining of human breast cancer using 11239-1-AP (same clone as 11239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cathepsin K Polyclonal antibody (11239-1-AP)

    IHC staining of human breast cancer using 11239-1-AP (same clone as 11239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cathepsin K Polyclonal antibody (11239-1-AP)

    IHC staining of human lung cancer using 11239-1-AP (same clone as 11239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cathepsin K Polyclonal antibody (11239-1-AP)

    IHC staining of human lung cancer using 11239-1-AP (same clone as 11239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cathepsin K Polyclonal antibody (11239-1-AP)

    IHC staining of human breast cancer using 11239-1-AP (same clone as 11239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11239-1-AP (Cathepsin K antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human osteosarcoma tissue using Cathepsin K Polyclonal antibody (11239-1-AP)

    IHC staining of human osteosarcoma using 11239-1-AP (same clone as 11239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:100. This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human osteosarcoma tissue using Cathepsin K Polyclonal antibody (11239-1-AP)

    IHC staining of human osteosarcoma using 11239-1-AP (same clone as 11239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:100. This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Cathepsin K Polyclonal antibody (11239-1-AP)

    IF Staining of mouse brain using 11239-1-AP (same clone as 11239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cathepsin K antibody (11239-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Cathepsin K Polyclonal antibody (11239-1-AP)

    IF Staining of mouse brain using 11239-1-AP (same clone as 11239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Cathepsin K antibody (11239-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of ROS1728 cells using Cathepsin K Polyclonal antibody (11239-1-AP)

    IF Staining of ROS1728 using 11239-1-AP (same clone as 11239-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed ROS1728 cells using Cathepsin K antibody (11239-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Cathepsin K Polyclonal antibody, PBS Only

Cathepsin K Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 11239-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

CTSK, Cathepsin X, catK, CTSO, CTSO2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11239-1-PBS targets Cathepsin K in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1761

Product name: Recombinant human CTSK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-329 aa of BC016058

Sequence: MWGLKVLLLPVVSFALYPEEILDTHWELWKKTHRKQYNNKVDEISRRLIWEKNLKYISIHNLEASLGVHTYELAMNHLGDMTSEEVVQKMTGLKVPLSHSRSNDTLYIPEWEGRAPDSVDYRKKGYVTPVKNQGQCGSCWAFSSVGALEGQLKKKTGKLLNLSPQNLVDCVSENDGCGGGYMTNAFQYVQKNRGIDSEDAYPYVGQEESCMYNPTGKAAKCRGYREIPEGNEKALKRAVARVGPVSVAIDASLTSFQFYSKGVYYDESCNSDNLNHAVLAVGYGIQKGNKHWIIKNSWGENWGNKGYILMARNKNNACGIANLASFPKM

相同性解析による交差性が予測される生物種
Full Name cathepsin K
Calculated molecular weight 39 kDa
Observed molecular weight27 kDa, 38~46 kDa
GenBank accession numberBC016058
Gene Symbol Cathepsin K
Gene ID (NCBI) 1513
RRIDAB_2245581
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP43235
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CTSK(cathepsin K), also named CTSO, CTSO2, is a recently identified lysosomal cysteine proteinase. CTSK is synthesized as a proenzyme of 38 kDa and subsequently enters acidic lysosomal compartments, in which the propeptide is cleaved and transformed into an active enzyme and it may influence adipocyte differentiation through modifying extracellular matrix components(PMID:16912123). It is revealed both the 46-kDa cathepsin-K precursor and the 30-kDa mature form in mouse bone extracts (PMID:9811821). The high CTSK protein levels are only detected in primary cultured fibroblasts derived from normal and neoplastic breast tissue, where the 37- and 25-kDa bands to be detected correspond to pro- and mature proteins through western blot (PMID:18765527). The full length protein has a signal peptides with 15 amino acids and a propeptide with 99 amino acids. CTSK may have cross reaction with the other members of cathepsin family and form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

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