Validation Data Gallery
Tested Applications
| Positive WB detected in | Calu-3 cells, mouse stomach tissue, rat stomach tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25146-1-AP targets Cathepsin E in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18484 Product name: Recombinant human CTSE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 108-275 aa of BC042537 Sequence: YCTSPACKTHSRFQPSQSSTYSQPGQSFSIQYGTGSLSGIIGADQVSVEGLTVVGQQFGESVTEPGQTFVDAEFDGILGLGYPSLAVGGVTPVFDNMMAQNLVDLPMFSVYMSSNPEGGAGSELIFGGYDHSHFSGSLNWVPVTKQAYWQIALDNIQVGGTVMFCSEG 相同性解析による交差性が予測される生物種 |
| Full Name | cathepsin E |
| Calculated molecular weight | 396 aa, 43 kDa |
| Observed molecular weight | 43-48 kDa |
| GenBank accession number | BC042537 |
| Gene Symbol | CTSE |
| Gene ID (NCBI) | 1510 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14091 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cathepsin E (CTSE) is an aspartic protease and a member of the peptidase A1 family of proteases, along with pepsin A and cathepsin D (CTSD) (PMID: 31582345). Caathepsin E is essential for degradation of these lysosomal membrane proteins, and that its deficiency is implicated in the development of a new form of lysosomal storage disorder associated with the elevation of lysosomal pH (PMID: 17095504). It associates with lysosomal and endosomal pathways participating actively in immune response regulation. Cathepsin E specifically induces growth arrest and apoptosis in human prostate cancer cell lines (PMID: 20482316).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cathepsin E antibody 25146-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |