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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cathepsin D Recombinant antibody (85964-1-RR)

    WB analysis using 85964-1-RR (same clone as 85964-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85964-1-RR (Cathepsin D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85964-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02204-2

    Sandwich ELISA standard curve of MP02204-2

    Sandwich ELISA standard curve of MP02204-2, Human Cathepsin D Recombinant Matched Antibody Pair - PBS only. 85964-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2986. 85964-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • Sample test of MP02204-2

    Sample test of MP02204-2

    The mean Cathepsin D concentration was determined to be 137.30 ng/mL in HepG2 cell extract based on a 0.90 mg/mL extract load.

  • Sample test of MP02204-2

    Sample test of MP02204-2

    Serum of nine mice was measured. The Cathepsin D concentration of detected samples was determined to be 1,136.75 ng/mL with a range of 773.75-1,505.92 pg/mL.

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Mouse Cathepsin D Recombinant antibody, PBS Only (Detector)

Cathepsin D Uni-rAbTM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85964-1-PBS
Clone No.250329A3

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Cathepsin D heavy chain, Cathepsin D light chain, CLN10, CPSD, CTSD

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85964-1-PBS targets Cathepsin D as part of a matched antibody pair:

MP02204-2: 85964-4-PBS capture and 85964-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cathepsin D fusion protein Eg2986 相同性解析による交差性が予測される生物種
Full Name cathepsin D
Calculated molecular weight45 kDa
Observed molecular weight46 kDa, 28 kDa
GenBank accession numberNM_009983.3
Gene Symbol Ctsd
Gene ID (NCBI) 13033
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18242
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CTSD (Cathepsin D) also named CPSD, is a representative aspartic proteinase in lysosomes and is widely distributed in various tissue cells of mammals. The expression level of CTSD varies depending on the tissue, whereas neurons in CNS tissues possess abundant CTSD. CTSD is synthesized as a 52-kDa precursor that is converted into an active ~48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 28-kDa heavy chain and a 14-kDa light chain, in the lysosomes (PMID: 10995834, PMID: 7641679, PMID: 30051532).