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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cathepsin B Recombinant monoclonal antibody (83998-4-RR)

    WB analysis using 83998-4-RR (same clone as 83998-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83998-4-RR (Cathepsin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83998-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03489-1

    Sandwich ELISA sample test of MP03489-1

    Urine of six individual healthy human donors was measured. The Cathepsin B concentration of detected samples was determined to be 1.64 ng/mL with a range of 0.76-2.39 ng/mL.

  • Sandwich ELISA sample test of MP03489-1

    Sandwich ELISA sample test of MP03489-1

    Saliva of six individual healthy human donors was measured. The Cathepsin B concentration of detected samples was determined to be 186.38 ng/mL with a range of 146.98-271.31 ng/mL.

  • Sandwich ELISA sample test of MP03489-1

    Sandwich ELISA sample test of MP03489-1

    Human milk of six individual healthy human donors was measured. The Cathepsin B concentration of detected samples was determined to be 150.72 ng/mL with a range of 95.82-192.06 ng/mL.

  • Sandwich ELISA sample test of MP03489-1

    Sandwich ELISA sample test of MP03489-1

    Plasma of six individual healthy human donors was measured. The Cathepsin B concentration of detected samples was determined to be 65.00 ng/mL with a range of 32.20-170.78 ng/mL.

  • Sandwich ELISA standard curve of MP03489-1

    Sandwich ELISA standard curve of MP03489-1

    Sandwich ELISA standard curve of MP03489-1, Human Cathepsin B Recombinant Matched Antibody Pair - PBS only. 83998-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1020. 83998-5-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Cathepsin B Recombinant monoclonal antibody (83998-4-RR)

    IP experiment of HepG2 using 83998-4-RR (same clone as 83998-4-PBS)

    IP result of anti-Cathepsin B (IP:83998-4-RR, 4ug; Detection:83998-4-RR 1:600) with HepG2 cells lysate 1280 ug. This data was developed using the same antibody clone with 83998-4-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03489-1

    Cytometric bead array sample test of MP03489-1

    The mean Cathepsin B concentration was determined to be 41.1 ng/mL in human plasma.

  • Cytometric bead array standard curve of MP03489-1

    Cytometric bead array standard curve of MP03489-1

    Cytometric bead array standard curve of MP03489-1, Cathepsin B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83998-4-PBS. Detection antibody: 83998-5-PBS. Standard: Eg1020. Range: 0.078-10 ng/mL.

Cathepsin B Recombinant monoclonal antibody, PBS Only (Capture)

Cathepsin B Uni-rAb® Recombinant Antibody for WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 83998-4-PBS
Clone No.260854C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

CTSB, APP secretase, APPS, Cat B, Cathepsin B heavy chain

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83998-4-PBS targets Cathepsin B as part of a matched antibody pair:

MP03489-1: 83998-4-PBS capture and 83998-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1020

Product name: Recombinant human Cathepsin B protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-10*His

Domain: 18-339 aa of NM_001908.5

Sequence: RSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI

相同性解析による交差性が予測される生物種
Full Name cathepsin B
Calculated molecular weight38kDa
Observed molecular weight31 kDa, 43 kDa
GenBank accession numberNM_001908.5
Gene Symbol Cathepsin B
Gene ID (NCBI) 1508
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP07858
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa mature forms in mouse brain by western blot(PMID:20616152).

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