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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Caspase 8/P43/P18 Polyclonal antibody (13423-1-AP)

    WB analysis of Jurkat using 13423-1-AP (same clone as 13423-1-PBS)

    Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of UV treated Hela using Caspase 8/P43/P18 Polyclonal antibody (13423-1-AP)

    WB analysis of UV treated Hela using 13423-1-AP (same clone as 13423-1-PBS)

    UV treated Hela cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW264.7 using Caspase 8/P43/P18 Polyclonal antibody (13423-1-AP)

    WB analysis of RAW264.7 using 13423-1-AP (same clone as 13423-1-PBS)

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Caspase 8/P43/P18 Polyclonal antibody (13423-1-AP)

    IP experiment of HeLa using 13423-1-AP (same clone as 13423-1-PBS)

    IP result of anti-Caspase 8/P43/P18 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using Caspase 8/P43/P18 Polyclonal antibody (13423-1-AP)

    IP experiment of Jurkat using 13423-1-AP (same clone as 13423-1-PBS)

    IP result of anti-Caspase 8/P43/P18 (IP:13423-1-AP, 4ug; Detection:13423-1-AP 1:500) with Jurkat cells lysate 2880 ug. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Caspase 8/P43/P18 Polyclonal antibody (13423-1-AP)

    IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Caspase 8/P43/P18 Polyclonal antibody (13423-1-AP)

    IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Caspase 8/P43/P18 Polyclonal antibody (13423-1-AP)

    IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Caspase 8/P43/P18 Polyclonal antibody (13423-1-AP)

    IHC staining of human colon using 13423-1-AP (same clone as 13423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Caspase 8/P43/P18 Polyclonal antibody (13423-1-AP)

    IHC staining of human colon using 13423-1-AP (same clone as 13423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Caspase 8/P43/P18 Polyclonal antibody (13423-1-AP)

    IF Staining of HepG2 using 13423-1-AP (same clone as 13423-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 8/P43/P18 antibody (13423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Caspase 8/P43/P18 Polyclonal antibody, PBS Only

Caspase 8/P43/P18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13423-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CASP8, Caspase 8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13423-1-PBS targets Caspase 8/P43/P18 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 8/P43/P18 fusion protein Ag4214 相同性解析による交差性が予測される生物種
Full Name caspase 8, apoptosis-related cysteine peptidase
Calculated molecular weight 538 aa, 62 kDa
Observed molecular weight 53-57 kDa, 32-45 kDa
GenBank accession numberBC028223
Gene Symbol Caspase 8
Gene ID (NCBI) 841
RRIDAB_2068463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14790
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.