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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Caspase 6 Recombinant antibody (85201-2-RR)

    WB analysis of Jurkat using 85201-2-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 85201-2-RR (Caspase 6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Caspase 6 Recombinant antibody

Caspase 6 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85201-2-RR
Clone No.242457A8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CASP6, Caspase6, 242457A8, Apoptotic protease Mch 2, Apoptotic protease Mch-2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85201-2-RR targets Caspase 6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name caspase 6, apoptosis-related cysteine peptidase
Calculated molecular weight 33 kDa, 22 kDa
Observed molecular weight33 kDa
GenBank accession numberBC000305
Gene Symbol Caspase 6
Gene ID (NCBI) 839
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP55212
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons.

Protocols

Product Specific Protocols
WB protocol for Caspase 6 antibody 85201-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols