Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 71 publications below |
| IHC | See 10 publications below |
| IF | See 14 publications below |
Product Information
31020-1-AP targets Caspase 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34441 Product name: Recombinant mouse Casp1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 119-296 aa of P29466 Sequence: DNPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFK 相同性解析による交差性が予測される生物種 |
| Full Name | caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | P29466 |
| Gene Symbol | Caspase 1 |
| Gene ID (NCBI) | 834 |
| RRID | AB_3669817 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P29466 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 1 antibody 31020-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine ErTao decoction alleviates liver fibrosis by suppressing STING-mediated macrophages and NLRP3 inflammasome activation | ||
Heliyon High-intensity interval training and moderate-intensity continuous training alleviate vascular dysfunction in spontaneously hypertensive rats through the inhibition of pyroptosis | ||
Physiol Res Trimethylamine Induced Chronic Kidney Injury by Activating the ZBP1-NLRP3 Inflammasome Pathway | ||
Free Radic Biol Med Senp1 knockdown alleviates neuronal pyroptosis after subarachnoid hemorrhage by enhancing PINK1-mediated mitophagy. | ||
J Dairy Sci Yogurt alleviates alcohol-induced hepatocyte pyroptosis and inflammation via autophagy/P62/Nrf2/ROS/NLRP3 pathway | ||
Burns Trauma Flufenamic acid inhibits pyroptosis in ischemic flaps via the AMPK-TRPML1-Calcineurin signaling pathway. |