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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Casein Kinase 1 Delta Polyclonal antibody (14388-1-AP)

    WB analysis using 14388-1-AP (same clone as 14388-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14388-1-AP (Casein Kinase 1 Delta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Casein Kinase 1 Delta Polyclonal antibody (14388-1-AP)

    WB analysis of HEK-293 using 14388-1-AP (same clone as 14388-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14388-1-AP (Casein Kinase 1 delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Casein Kinase 1 Delta Polyclonal antibody (14388-1-AP)

    IP experiment of HeLa using 14388-1-AP (same clone as 14388-1-PBS)

    IP result of anti-Casein Kinase 1 Delta (IP:14388-1-AP, 3ug; Detection:14388-1-AP 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Casein Kinase 1 Delta Polyclonal antibody (14388-1-AP)

    IHC staining of mouse heart using 14388-1-AP (same clone as 14388-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14388-1-AP (Casein Kinase 1 Delta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Casein Kinase 1 Delta Polyclonal antibody (14388-1-AP)

    IHC staining of mouse brain using 14388-1-AP (same clone as 14388-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14388-1-AP (Casein Kinase 1 Delta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Casein Kinase 1 Delta Polyclonal antibody (14388-1-AP)

    IF Staining of HepG2 using 14388-1-AP (same clone as 14388-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Casein Kinase 1 Delta antibody (14388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using Casein Kinase 1 Delta Polyclonal antibody (14388-1-AP)

    IF Staining of U-251 using 14388-1-AP (same clone as 14388-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using Casein Kinase 1 Delta antibody (14388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Casein Kinase 1 Delta Polyclonal antibody, PBS Only

Casein Kinase 1 Delta Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14388-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14388-1-PBS targets Casein Kinase 1 Delta in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5856

Product name: Recombinant human CSNK1D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 103-373 aa of BC003558

Sequence: KTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGASRAADDAERERRDREERLRHSRNPATRGLPSTASGRLRGTQEVAPPTPLTPTSHTANTSPRPVSGMERERKVSMRL

相同性解析による交差性が予測される生物種
Full Name casein kinase 1, delta
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC003558
Gene Symbol CK1 Delta
Gene ID (NCBI) 1453
RRIDAB_2084484
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP48730
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.

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