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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cas9 Recombinant antibody (85389-1-RR)

    WB analysis using 85389-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85389-1-RR (Cas9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85389-1-RR

    Affinity and Kinetic Characterization of 85389-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85389-1-RR against Human Cas9 were performed. The affinity constant is 3.18 nM.

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Cas9 Recombinant antibody

Cas9 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85389-1-RR
Clone No.242781G12

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

WB, ELISA

Synonyms

242781G12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85389-1-RR targets Cas9 in WB, ELISA applications and shows reactivity with n/a samples.

Tested Reactivity n/a
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cas9 fusion protein Ag25281 相同性解析による交差性が予測される生物種
Full Name Cas9
Calculated molecular weight 160 kDa
GenBank accession numberNP_269215
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes. This antibody detects the sp Cas9.

Protocols

Product Specific Protocols
WB protocol for Cas9 antibody 85389-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols