Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, human adipose tissue, human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87661-1-RR targets Carboxypeptidase M in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg5655 Product name: Recombinant human CBPM protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 18-423 aa of NM_198320.5 Sequence: LDFNYHRQEGMEAFLKTVAQNYSSVTHLHSIGKSVKGRNLWVLVVGRFPKEHRIGIPEFKYVANMHGDETVGRELLLHLIDYLVTSDGKDPEITNLINSTRIHIMPSMNPDGFEAVKKPDCYYSIGRENYNQYDLNRNFPDAFEYNNVSRQPETVAVMKWLKTETFVLSANLHGGALVASYPFDNGVQATGALYSRSLTPDDDVFQYLAHTYASRNPNMKKGDECKNKMNFPNGVTNGYSWYPLQGGMQDYNYIWAQCFEITLELSCCKYPREEKLPSFWNNNKASLIEYIKQVHLGVKGQVFDQNGNPLPNVIVEVQDRKHICPYRTNKYGEYYLLLLPGSYIINVTVPGHDPHITKVIIPEKSQNFSALKKDILLPFQGQLDSIPVSNPSCPMIPLYRNLPDHS 相同性解析による交差性が予測される生物種 |
| Full Name | carboxypeptidase M |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 51 kDa, 62-70 kDa |
| GenBank accession number | NM_198320.5 |
| Gene Symbol | Carboxypeptidase M |
| Gene ID (NCBI) | 1368 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P14384 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CPM(Carboxypeptidase M), a plasma membrane-bound enzyme, is present in many human organs and differs from other carboxypeptidases that cleave basic C-terminal amino acids(PMID:2394713). CPM is structurally, catalytically and immunologically distinct from other carboxypeptidases, and expressed on cell membranes in a glycosyl-phosphatidylinositol-anchored form and it can be a novel marker and cellular player in lipid uptake and/or metabolism of MAs by promoting foam cell formation (PMID:22157720). This full length protein has a signal peptide with 17 amino acid, a propeptide with 20 amino acid and five glycosylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Carboxypeptidase M antibody 87661-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |