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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human kidney(renal cell) cancer using Carbonic Anhydrase IX/CA9 Recombinant monoclonal a (84233-8-RR)

    IHC staining of human kidney(renal cell) cancer using 84233-8-RR (same clone as 84233-8-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell cancer slide using 84233-8-RR (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-8-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney(renal cell) cancer using Carbonic Anhydrase IX/CA9 Recombinant monoclonal a (84233-8-RR)

    IHC staining of human kidney(renal cell) cancer using 84233-8-RR (same clone as 84233-8-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell cancer slide using 84233-8-RR (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-8-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Carbonic Anhydrase IX/CA9 Recombinant monoclonal a (84233-8-RR)

    IHC staining of human kidney using 84233-8-RR (same clone as 84233-8-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative control) slide using 84233-8-RR (CA9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-8-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Carbonic Anhydrase IX/CA9 Recombinant monoclonal a (84233-8-RR)

    IHC staining of human kidney using 84233-8-RR (same clone as 84233-8-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative control) slide using 84233-8-RR (CA9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-8-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Carbonic Anhydrase IX/CA9 Recombinant monoclonal a (84233-8-RR)

    IHC staining of human renal cell carcinoma using 84233-8-RR (same clone as 84233-8-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84233-8-RR (CA9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-8-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Carbonic Anhydrase IX/CA9 Recombinant monoclonal a (84233-8-RR)

    IHC staining of human renal cell carcinoma using 84233-8-RR (same clone as 84233-8-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84233-8-RR (CA9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-8-PBS in a different storage buffer formulation.

  • Affinity of 84233-8-RR

    Affinity of 84233-8-RR

    Biolayer interferometry (BLl) kinetic assays of 84233-8-RR against Human Carbonic Anhydrase IX/CA9 were performed. The affinity constant is 0.469 nM.

Carbonic Anhydrase IX/CA9 Recombinant monoclonal antibody, PBS Only

Carbonic Anhydrase IX/CA9 Uni-rAb® Recombinant Antibody for IHC, Indirect ELISA
Cat No. 84233-8-PBS
Clone No.241573F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Synonyms

CA9, CAIX, carbonic anhydrase IX, CA 9, CA IX

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84233-8-PBS targets Carbonic Anhydrase IX/CA9 in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag36941

Product name: Recombinant human CA9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 32-408 aa of BC014950

Sequence: LMPVHPQRLPRMQEDSPLGGGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNS

相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase IX
Calculated molecular weight 459 aa, 50 kDa
GenBank accession numberBC014950
Gene Symbol CA9
Gene ID (NCBI) 768
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ16790
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. The protein is present in the cytoplasmic membrane and the nucleus, with a molecular weight of 50 kDa. The molecular weight of the glycosylated form is approximately 60-70 kDa. (PMID: 31142270, PMID: 31819036)

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