Recombinant Canine SCF protein (rFc Tag)
Species
Canine
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg4548
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Canine SCF protein Lys26-Gln215 (Accession# Q06220) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 403507 |
| Accession | Q06220 |
| PredictedSize | 47.3 kDa |
| SDS-PAGE | 50-65 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Stem cell factor (SCF), also known as mast cell growth factor, steel factor and Kit ligand, is a haematopoietic growth factor. SCF is a critical protein with key roles in the cell such as haematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signalling pathways by binding to the c-kit receptor. SCF and its receptor, c-kit ligand (KL), are particularly upregulated in human malignancies, including gastrointestinal stromal tumours (GISTs), breast cancer, haematopoietic malignancies, myeloid leukaemia and gliomas. The SCF/c-KIT system also plays an important role in cell fate decision and its activity is known to control cell proliferation, differentiation, survival and apoptosis.
References:
1.Vazquez-Mellado MJ. et al. (2017) Vitam Horm. 105:273-296. 2.Figueira MI. et al. (2017) Cancer Lett. 405:10-21. 3.Yasuda A. et al. (2006) Mol Cancer. 5:46.