Recombinant Canine IL-2 protein (rFc Tag) (HPLC verified)
Species
Canine
Purity
>90 %, SDS-PAGE
>90%, SEC-HPLC
Tag
rFc Tag
Activity
not tested
Cat no : Eg4556
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE >90%, SEC-HPLC |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Canine IL-2 protein Ala21-Thr155 (Accession# Q29416) with a rabbit IgG Fc tag at the N-terminus. |
| GeneID | 403989 |
| Accession | Q29416 |
| PredictedSize | 42.7 kDa |
| SDS-PAGE | 42-50 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Interleukin-2 (IL-2) is a protein originally identified as a T-cell growth factor. Il2 is mainly produced by activated CD4+CD25- (helper) T cells and induces the proliferation of these and other cells such as CD8+ T cells, B and NK cells. For this reason, it has been used to treat immune deficiencies such as HIV and to induce immune responses against tumours. However, IL-2 has also been shown to be a major growth factor for CD4+CD25+ regulatory T cells. Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (Il2R).
References:
1.Boyman O. et al. (2012) Nat Rev Immunol. 12(3):180-90. 2.Raeber ME. et al. (2023) EBioMedicine. 90:104539. 3.Malek TR. (2008) Annu Rev Immunol. 26:453-79.