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Validation Data Gallery

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  • Western Blot (WB) analysis of human stomach tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    WB analysis of human stomach using 17556-1-AP (same clone as 17556-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human stomach tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    WB analysis of human stomach using 17556-1-AP (same clone as 17556-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    WB analysis of mouse small intestine using 17556-1-AP (same clone as 17556-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using Calpain 9 Polyclonal antibody (17556-1-AP)

    WB analysis of HL-60 using 17556-1-AP (same clone as 17556-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human stomach tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    WB analysis of human stomach using 17556-1-AP (same clone as 17556-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human stomach cancer using 17556-1-AP (same clone as 17556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human stomach cancer using 17556-1-AP (same clone as 17556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human stomach using 17556-1-AP (same clone as 17556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human stomach using 17556-1-AP (same clone as 17556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human kidney using 17556-1-AP (same clone as 17556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human kidney using 17556-1-AP (same clone as 17556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human testis using 17556-1-AP (same clone as 17556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human testis using 17556-1-AP (same clone as 17556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human lung using 17556-1-AP (same clone as 17556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human lung using 17556-1-AP (same clone as 17556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human ovary using 17556-1-AP (same clone as 17556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human ovary using 17556-1-AP (same clone as 17556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Calpain 9 Polyclonal antibody (17556-1-AP)

    IF Staining of HepG2 using 17556-1-AP (same clone as 17556-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using CAPN9 antibody 17556-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 17556-1-PBS in a different storage buffer formulation.

Calpain 9 Polyclonal antibody, PBS Only

Calpain 9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17556-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17556-1-PBS targets Calpain 9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11718

Product name: Recombinant human CAPN9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 178-627 aa of BC027993

Sequence: ESEARTPFGLIKGHAYSVTGIDQVSFRGQRIELIRIRNPWGQVEWNGSWSDSSPEWRSVGPAEQKRLCHTALDDGEFWMAFQDFKAHFDKVEICNLTPDALEEDAIHKWEVTVHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSLTEKDEGQEECSFLVALMQKDRRKLKRFGANVLTIGYAIYECPDKDEHLNKDFFRYHASRARSKTFINLREVSDRFKLPPGEYILIPSTFEPHQEADFCLRIFSEKKAITRDMDGNVDIDLPEPPKPTPPDQETEEEQRFRALFEQVAGEDMEVTAEELEYVLNAVLQKKKDIKFKKLSLISCKNIISLMDTSGNGKLEFDEFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYADEELQLDFDDFLNCLVRLENASRVFQALSTKNKEFIHLNINEFIHLTMNI

相同性解析による交差性が予測される生物種
Full Name calpain 9
Calculated molecular weight 627 aa, 72 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC027993
Gene Symbol Calpain 9
Gene ID (NCBI) 10753
RRIDAB_2290833
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14815
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Calpain 9 is also named as NCL4 and belongs to the peptidase C2 family.It can be down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types.It is predominantly expressed in the gastrointestinal tract and restricted to the gastric surface mucus (pit) cells in the stomach and the function of it is implicated in suppressing tumorigenesis(PMID:20686710).

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