Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33924-1-AP targets Calpain 15/SOLH in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag40689 Product name: Recombinant human SOLH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC021854 Sequence: MATVGEWSCVRCTFLNPAGQRQCSICEAPRHKPDLNHILRLSVEEQKWPCARCTFRNFLGKEACEVCGFTPEPAPGAAFLPVLNGVLPKPPAILGEPKGSCQEEAGPVRTAGLVATEPARGQCEDKDEEEKEEQEEEEGAAEPRGGWACP 相同性解析による交差性が予測される生物種 |
| Full Name | small optic lobes homolog (Drosophila) |
| Calculated molecular weight | 117 kDa |
| Observed molecular weight | 117 kDa |
| GenBank accession number | BC021854 |
| Gene Symbol | SOLH |
| Gene ID (NCBI) | 6650 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O75808 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Calpain 15 (also known as CAPN15 and SOLH) is an intracellular cysteine protease belonging to the non-classical small optic lobe (SOL) family of calpains, which plays an important role in developmental processes (PMID: 40238785). Capn15 is expressed at much higher levels during early neuronal development than in the adult (PMID: 32885237). And loss of Capn15 in forebrain excitatory neurons leads to some behavioral deficits including decreased fear generalization (PMID: 37598906).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Calpain 15/SOLH antibody 33924-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |