Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
13675-1-PBS targets Calpain 11 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4668 Product name: Recombinant human CAPN11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 352-702 aa of BC033733 Sequence: YWHTTFYEGSWRRGSSAGGCRNHPGTFWTNPQFKISLPEGDDPEDDAEGNVVACTCLVALMQKNWRHARQQGAQLQTIGFVLYAVPKEFQNIQDVHLKKEFFTKYQDHGFSEIFTNSREVSSQLRLPPGEYIIIPSTFEPHRDADFLLRVFTEKHSESWELDEVNYAEQLQEEKVSEDDMDQDFLHLFKIVAGEGKEIGVYELQRLLNRMAIKFKSFKTKGFGLDACRCMINLMDKDGSGKLGLLEFKILWKKLKKWMDIFRECDQDHSGTLNSYEMRLVIEKAGIKLNNKVMQVLVARYADDDLIIDFDSFISCFLRLKTMFTFFLTMDPKNTGHICLSLEQWLQMTMWG 相同性解析による交差性が予測される生物種 |
| Full Name | calpain 11 |
| Calculated molecular weight | 702 aa, 81 kDa |
| Observed molecular weight | 81 kDa |
| GenBank accession number | BC033733 |
| Gene Symbol | Calpain 11 |
| Gene ID (NCBI) | 11131 |
| RRID | AB_2069480 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UMQ6 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Calpains are a superfamily of related proteins, some of which have been shown to function as intracellular calcium-dependent cysteine proteases. Calpains consist of a variable large (80 kDa) subunit and an invariant small (30 kDa) subunit. The expression of calpain11 during spermatogenesis and its localization is spermatozoa suggest that it is involved in regulating calcium-dependent signal transduction events during meiosis and sperm functional processes(PMID:16541461). And the 110 kDa band protein may be regarded as a complex.