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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calmodulin 1/2/3 Polyclonal antibody (28270-1-AP)

    WB analysis using 28270-1-AP (same clone as 28270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Calmodulin 1/2/3 Polyclonal antibody (28270-1-AP)

    IHC staining of rat brain using 28270-1-AP (same clone as 28270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Calmodulin 1/2/3 Polyclonal antibody (28270-1-AP)

    IHC staining of rat brain using 28270-1-AP (same clone as 28270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Calmodulin 1/2/3 Polyclonal antibody (28270-1-AP)

    IHC staining of mouse brain using 28270-1-AP (same clone as 28270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Calmodulin 1/2/3 Polyclonal antibody (28270-1-AP)

    IHC staining of mouse skeletal muscle using 28270-1-AP (same clone as 28270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28270-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Calmodulin 1/2/3 Polyclonal antibody (28270-1-AP)

    IF Staining of HeLa using 28270-1-AP (same clone as 28270-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calmodulin 1/2/3 antibody (28270-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28270-1-PBS in a different storage buffer formulation.

Calmodulin 1/2/3 Polyclonal antibody, PBS Only

Calmodulin 1/2/3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28270-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CALM2, CALM, CALM1, CALM3, CALML2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28270-1-PBS targets Calmodulin 1/2/3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag28000

Product name: Recombinant human CALM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 72-149 aa of BC006464

Sequence: MMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK

相同性解析による交差性が予測される生物種
Full Name calmodulin 2 (phosphorylase kinase, delta)
Calculated molecular weight 17 kDa
Observed molecular weight 17-22 kDa
GenBank accession numberBC006464
Gene Symbol Calmodulin 1
Gene ID (NCBI) 805
RRIDAB_3669650
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP62158
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Calmodulin is the most widely distributed and most versatile member of a family of calcium-binding proteins which probably serve as receptors for the Ca2+ signal. Through the regulation of a wide variety of intracellular enzymes, calmodulin plays a key role in many physiological processes (PMID: 6313166).

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