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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using Calmegin Polyclonal antibody (12629-1-AP)

    WB analysis of mouse testis using 12629-1-AP (same clone as 12629-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Calmegin Polyclonal antibody (12629-1-AP)

    WB analysis of mouse brain using 12629-1-AP (same clone as 12629-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Calmegin Polyclonal antibody (12629-1-AP)

    WB analysis of human brain using 12629-1-AP (same clone as 12629-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using Calmegin Polyclonal antibody (12629-1-AP)

    WB analysis of mouse lung using 12629-1-AP (same clone as 12629-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human lung tissue using Calmegin Polyclonal antibody (12629-1-AP)

    WB analysis of human lung using 12629-1-AP (same clone as 12629-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using Calmegin Polyclonal antibody (12629-1-AP)

    IP experiment of mouse testis using 12629-1-AP (same clone as 12629-1-PBS)

    IP result of anti-Calmegin (IP:12629-1-AP, 4ug; Detection:12629-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Calmegin Polyclonal antibody (12629-1-AP)

    IHC staining of human testis using 12629-1-AP (same clone as 12629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12629-1-AP (Calmegin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Calmegin Polyclonal antibody (12629-1-AP)

    IHC staining of human testis using 12629-1-AP (same clone as 12629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12629-1-AP (Calmegin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

Calmegin Polyclonal antibody, PBS Only

Calmegin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12629-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12629-1-PBS targets Calmegin in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3322

Product name: Recombinant human CLGN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 311-610 aa of BC028357

Sequence: PAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPACRIGCGEWKPPMIDNPKYKGVWRPPLVDNPNYQGIWSPRKIPNPDYFEDDHPFLLTSFSALGLELWSMTSDIYFDNFIICSEKEVADHWAADGWRWKIMIANANKPGVLKQLMAAAEGHPWLWLIYLVTAGVPIALITSFCWPRKVKKKHKDTEYKKTDICIPQTKGVLEQEEKEEKAALEKPMDLEEEKKQNDGEMLEKEEESEPEEKSEEEIEIIEGQEESNQSNKSGSEDEMKEADESTGSGDGPIKSVRKRRVRKD

相同性解析による交差性が予測される生物種
Full Name calmegin
Calculated molecular weight 610 aa, 70 kDa
Observed molecular weight 93 kDa
GenBank accession numberBC028357
Gene Symbol Calmegin
Gene ID (NCBI) 1047
RRIDAB_2276364
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14967
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Calmegin, belonging to the calreticulin family, is a testis-specific molecular chaperon of the endoplasmic reticulum playing a key role in spermatogenesis. Western blot analysis of mouse testis showed a single band with an apparent molecular mass of 93 kDa.

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