Validation Data Gallery
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Tested Applications
| Positive WB detected in | NIH/3T3 cells, HeLa cells, mouse brain tissue, Neuro-2a cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
25390-1-AP targets CYTSA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CYTSA fusion protein Ag21896 相同性解析による交差性が予測される生物種 |
| Full Name | cytospin A |
| Calculated molecular weight | 1117 aa, 125 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC136471 |
| Gene Symbol | CYTSA |
| Gene ID (NCBI) | 23384 |
| RRID | AB_2880056 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q69YQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CYTSA, also named as SPECC1L or KIAA0376, is a 1117 amino acid protein, which contains one CH domain and belongs to the cytospin-A family. CYTSA localizes in the cytoplasm and is involved in cytokinesis and spindle organization. CYTSA may play a role in actin cytoskeleton organization and microtubule stabilization, hence it is required for proper cell adhesion and migration.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYTSA antibody 25390-1-AP | Download protocol |
| IHC protocol for CYTSA antibody 25390-1-AP | Download protocol |
| IP protocol for CYTSA antibody 25390-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet In-frame deletion of SPECC1L microtubule association domain results in gain-of-function phenotypes affecting embryonic tissue movement and fusion events. | ||
Curr Biol EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules | ||
J Biol Chem SPECC1L binds the myosin phosphatase complex MYPT1/PP1β and can regulate its distribution between microtubules and filamentous actin |