Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, SMMC-7721 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84909-1-RR targets CYP8B1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 8, subfamily B, polypeptide 1 |
| Calculated molecular weight | 58 kDa |
| Observed molecular weight | 50-58 kDa |
| GenBank accession number | NM_004391 |
| Gene Symbol | CYP8B1 |
| Gene ID (NCBI) | 1582 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9UNU6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytochrome P450 8B1 (CYP8B1) , also named as CP8B, CYP12 and CYPVIIIB1, is an enzyme required for the synthesis of cholic acid (CA) in the classic bile acid synthesis pathway. CYP8B1 controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP8B1 antibody 84909-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |