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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP8B1 Polyclonal antibody (55217-1-AP)

    WB analysis using 55217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55217-1-AP (CYP8B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CYP8B1 Polyclonal antibody (55217-1-AP)

    WB analysis using 55217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55217-1-AP (CYP8B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CYP8B1 Polyclonal antibody (55217-1-AP)

    IHC staining of mouse liver using 55217-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55217-1-AP (CYP8B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CYP8B1 Polyclonal antibody (55217-1-AP)

    IF Staining of HepG2 using 55217-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP8B1 antibody (55217-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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CYP8B1 Polyclonal antibody

CYP8B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55217-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CP8B, CYP12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 450 ug/ml
20 μL, 450 ug/ml
150 μL, 450 ug/ml

¥67,000/ 150 μL

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, SMMC-7721 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55217-1-AP targets CYP8B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 8, subfamily B, polypeptide 1
Calculated molecular weight 58 kDa
Observed molecular weight 50-58 kDa
GenBank accession numberNM_004391
Gene Symbol CYP8B1
Gene ID (NCBI) 1582
RRIDAB_3670243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNU6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochrome P450 8B1 (CYP8B1) , also named as CP8B, CYP12 and CYPVIIIB1, is an enzyme required for the synthesis of cholic acid (CA) in the classic bile acid synthesis pathway. CYP8B1 controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids.

Protocols

Product Specific Protocols
WB protocol for CYP8B1 antibody 55217-1-APDownload protocol
IHC protocol for CYP8B1 antibody 55217-1-APDownload protocol
IF protocol for CYP8B1 antibody 55217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols