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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CYP7B1 Recombinant antibody (82927-2-RR)

    IF Staining of HepG2 using 82927-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7B1 antibody (82927-2-RR, Clone: 230160C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CYP7B1 Recombinant antibody (82927-2-RR)

    IF Staining of HeLa using 82927-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYP7B1 antibody (82927-2-RR, Clone: 230160C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CYP7B1 Recombinant antibody (82927-2-RR)

    IF Staining of HEK-293 using 82927-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (82927-2-RR, Clone: 230160C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using CYP7B1 Recombinant antibody (82927-2-RR)

    FC experiment of HeLa using 82927-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CYP7B1 Recombinant antibody (82927-2-RR, Clone:230160C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CYP7B1 Recombinant monoclonal antibody

CYP7B1 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82927-2-RR
Clone No.230160C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

230160C7, 24-hydroxycholesterol 7-alpha-hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 3-hydroxysteroid 7-alpha hydroxylase, CBAS3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells, HEK-293 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82927-2-RR targets CYP7B1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19374

Product name: Recombinant human CYP7B1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 96-403 aa of BC136574

Sequence: PFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGD

相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 7, subfamily B, polypeptide 1
Calculated molecular weight 506 aa, 58 kDa
GenBank accession numberBC136574
Gene Symbol CYP7B1
Gene ID (NCBI) 9420
RRIDAB_3670666
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO75881
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CYP7B1 antibody 82927-2-RRDownload protocol
IF protocol for CYP7B1 antibody 82927-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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