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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP7B1 Recombinant monoclonal antibody (82927-1-RR)

    WB analysis using 82927-1-RR (same clone as 82927-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82927-1-RR (CYP7B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CYP7B1 Recombinant monoclonal antibody (82927-1-RR)

    IF Staining of HepG2 using 82927-1-RR (same clone as 82927-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7B1 antibody (82927-1-RR, Clone: 230160H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CYP7B1 Recombinant monoclonal antibody (82927-1-RR)

    IF Staining of HEK-293 using 82927-1-RR (same clone as 82927-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (82927-1-RR, Clone: 230160H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using CYP7B1 Recombinant monoclonal antibody (82927-1-RR)

    FC experiment of HeLa using 82927-1-RR (same clone as 82927-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CYP7B1 Recombinant antibody (82927-1-RR, Clone:230160H5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

CYP7B1 Recombinant monoclonal antibody, PBS Only

CYP7B1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82927-1-PBS
Clone No.230160H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

230160H5, 24-hydroxycholesterol 7-alpha-hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 3-hydroxysteroid 7-alpha hydroxylase, CBAS3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82927-1-PBS targets CYP7B1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19374

Product name: Recombinant human CYP7B1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 96-403 aa of BC136574

Sequence: PFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGD

相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 7, subfamily B, polypeptide 1
Calculated molecular weight 506 aa, 58 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC136574
Gene Symbol CYP7B1
Gene ID (NCBI) 9420
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO75881
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.

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