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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using CYP7B1 Polyclonal antibody (24889-1-AP)

    WB analysis of A431 using 24889-1-AP (same clone as 24889-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CYP7B1 Polyclonal antibody (24889-1-AP)

    WB analysis using 24889-1-AP (same clone as 24889-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CYP7B1 Polyclonal antibody (24889-1-AP)

    WB analysis of HEK-293 using 24889-1-AP (same clone as 24889-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using CYP7B1 Polyclonal antibody (24889-1-AP)

    WB analysis of mouse liver using 24889-1-AP (same clone as 24889-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CYP7B1 Polyclonal antibody (24889-1-AP)

    IHC staining of mouse liver using 24889-1-AP (same clone as 24889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP7B1 Polyclonal antibody (24889-1-AP)

    IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP7B1 Polyclonal antibody (24889-1-AP)

    IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CYP7B1 Polyclonal antibody (24889-1-AP)

    IHC staining of mouse liver using 24889-1-AP (same clone as 24889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP7B1 Polyclonal antibody (24889-1-AP)

    IHC staining of human liver using 24889-1-AP (same clone as 24889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP7B1 Polyclonal antibody (24889-1-AP)

    IHC staining of human liver using 24889-1-AP (same clone as 24889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP7B1 Polyclonal antibody (24889-1-AP)

    IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP7B1 Polyclonal antibody (24889-1-AP)

    IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CYP7B1 Polyclonal antibody (24889-1-AP)

    IHC staining of human brain using 24889-1-AP (same clone as 24889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CYP7B1 Polyclonal antibody (24889-1-AP)

    IHC staining of human brain using 24889-1-AP (same clone as 24889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CYP7B1 Polyclonal antibody (24889-1-AP)

    IF Staining of HEK-293 using 24889-1-AP (same clone as 24889-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CYP7B1 Polyclonal antibody (24889-1-AP)

    IF Staining of HeLa using 24889-1-AP (same clone as 24889-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CYP7B1 Polyclonal antibody (24889-1-AP)

    IF Staining of HEK-293 using 24889-1-AP (same clone as 24889-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

CYP7B1 Polyclonal antibody, PBS Only

CYP7B1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24889-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

24-hydroxycholesterol 7-alpha-hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 3-hydroxysteroid 7-alpha hydroxylase, CBAS3, CP7B

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24889-1-PBS targets CYP7B1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19374

Product name: Recombinant human CYP7B1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 96-403 aa of BC136574

Sequence: PFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGD

相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 7, subfamily B, polypeptide 1
Calculated molecular weight 506 aa, 58 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC136574
Gene Symbol CYP7B1
Gene ID (NCBI) 9420
RRIDAB_2879780
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75881
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.

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