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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using CYP4F3 Polyclonal antibody (19701-1-AP)

    WB analysis of HL-60 using 19701-1-AP (same clone as 19701-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19701-1-AP (CYP4F3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 19701-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using CYP4F3 Polyclonal antibody (19701-1-AP)

    IHC staining of human ovarian cancer using 19701-1-AP (same clone as 19701-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 19701-1-AP (CYP4F3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19701-1-PBS in a different storage buffer formulation.

CYP4F3 Polyclonal antibody, PBS Only

CYP4F3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 19701-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, CPF3, CYP4F, CYPIVF3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19701-1-PBS targets CYP4F3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 4, subfamily F, polypeptide 3
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberNM_000896
Gene Symbol CYP4F3
Gene ID (NCBI) 4051
RRIDAB_2878599
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08477
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CYP4F3, also named as LTB4H and CYPIVF3, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F3 requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes.

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