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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP4F2-Specific Recombinant antibody (85320-1-RR)

    WB analysis using 85320-1-RR (same clone as 85320-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CYP4F2-Specific Recombinant antibody (85320-1-RR)

    WB analysis using 85320-1-RR (same clone as 85320-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP4F2-Specific Recombinant antibody (85320-1-RR)

    IHC staining of human liver cancer using 85320-1-RR (same clone as 85320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP4F2-Specific Recombinant antibody (85320-1-RR)

    IHC staining of human liver cancer using 85320-1-RR (same clone as 85320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Hepatocellular Cancer using CYP4F2-Specific Recombinant antibody (85320-1-RR)

    IHC staining of Human Hepatocellular Cancer using 85320-1-RR (same clone as 85320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Hepatocellular Cancer using CYP4F2-Specific Recombinant antibody (85320-1-RR)

    IHC staining of Human Hepatocellular Cancer using 85320-1-RR (same clone as 85320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

CYP4F2-Specific Recombinant monoclonal antibody, PBS Only

CYP4F2-Specific Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85320-1-PBS
Clone No.242307B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

CYP4F2, 20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, 242307B10, Arachidonic acid omega-hydroxylase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85320-1-PBS targets CYP4F2-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 4, subfamily F, polypeptide 2
Calculated molecular weight 60 kDa
Observed molecular weight 50-53 kDa
GenBank accession numberNM_001082
Gene Symbol CYP4F2
Gene ID (NCBI) 8529
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP78329
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548).

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