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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP3A7 Polyclonal antibody

CYP3A7 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55428-1-AP

Synonyms

CP37, CYP3A7, CYPIIIA7, Cytochrome P450 3A7, Cytochrome P450 HFLA, P450 HFLA

Validation Data Gallery

Applications:
  • WB analysis of L02 using 55428-1-AP

    WB analysis of L02 using 55428-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 55428-1-AP (CYP3A7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 55428-1-AP

    WB analysis of HepG2 using 55428-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55428-1-AP (CYP3A7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 55428-1-AP

    WB analysis of HepG2 using 55428-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55428-1-AP (CYP3A7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 55428-1-AP

    IHC staining of human liver cancer using 55428-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55428-1-AP (CYP3A7 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 55428-1-AP

    IHC staining of human liver cancer using 55428-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55428-1-AP (CYP3A7 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 55428-1-AP

    IF Staining of HepG2 using 55428-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 55428-1-AP (CYP3A7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inL02 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

55428-1-AP targets CYP3A7 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 3, subfamily A, polypeptide 7
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_000765
Gene symbol CYP3A7
Gene ID (NCBI) 1551
RRIDAB_2881328
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYP3A7 antibody 55428-1-APDownload protocol
IHC protocol for CYP3A7 antibody 55428-1-APDownload protocol
IF protocol for CYP3A7 antibody 55428-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Front Bioeng Biotechnol

Recapitulation of Human Embryonic Heartbeat to Promote Differentiation of Hepatic Endoderm to Hepatoblasts.

Authors - Koki Yoshimoto
View Article