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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP39A1 Polyclonal antibody

CYP39A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16062-1-AP

Synonyms

CYP39A1, Cytochrome P450 39A1, hCYP39A1, Oxysterol 7 alpha hydroxylase

Validation Data Gallery

Applications:
  • WB analysis of L02 using 16062-1-AP

    WB analysis of L02 using 16062-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16062-1-AP (CYP39A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of SMMC-7721 using 16062-1-AP

    WB analysis of SMMC-7721 using 16062-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16062-1-AP (CYP39A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16062-1-AP

    IHC staining of human liver using 16062-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16062-1-AP (CYP39A1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 16062-1-AP

    IHC staining of human liver using 16062-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16062-1-AP (CYP39A1 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human liver cancer using 16062-1-AP

    IHC staining of human liver cancer using 16062-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16062-1-AP (CYP39A1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 16062-1-AP

    IHC staining of human liver cancer using 16062-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16062-1-AP (CYP39A1 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inL02 cells, SMMC-7721 cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16062-1-AP targets CYP39A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP39A1 fusion protein Ag8896 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 39, subfamily A, polypeptide 1
Calculated molecular weight 469 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC010358
Gene symbol CYP39A1
Gene ID (NCBI) 51302
RRIDAB_2878211
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYP39A1 antibody 16062-1-APDownload protocol
IHC protocol for CYP39A1 antibody 16062-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols