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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP2F1 Polyclonal antibody

CYP2F1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21579-1-AP

Synonyms

C2F1, CYP2F, CYP2F1, CYPIIF1, Cytochrome P450 2F1

Validation Data Gallery

Applications:
  • WB analysis of mouse lung using 21579-1-AP

    WB analysis of mouse lung using 21579-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21579-1-AP (CYP2F1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 21579-1-AP

    WB analysis of mouse lung using 21579-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21579-1-AP (CYP2F1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 21579-1-AP

    IHC staining of human lung cancer using 21579-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21579-1-AP (CYP2F1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 21579-1-AP

    IHC staining of human lung cancer using 21579-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21579-1-AP (CYP2F1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21579-1-AP is CYP2F1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP2F1 fusion protein Ag16124 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily F, polypeptide 1
Calculated molecular weight 491 aa, 56 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC109056
Gene symbol CYP2F1
Gene ID (NCBI) 1572
RRIDAB_10733220
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYP2F1 antibody 21579-1-APDownload protocol
IHC protocol for CYP2F1 antibody 21579-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols