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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP2C9 Polyclonal antibody (16355-1-AP)

    WB analysis using 16355-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16355-1-AP (CYP2C9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using CYP2C9 Polyclonal antibody (16355-1-AP)

    WB analysis of SMMC-7721 using 16355-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16355-1-AP (CYP2C9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP2C9 Polyclonal antibody (16355-1-AP)

    IHC staining of human liver using 16355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16355-1-AP (CYP2C9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP2C9 Polyclonal antibody (16355-1-AP)

    IHC staining of human liver using 16355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16355-1-AP (CYP2C9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CYP2C9 Polyclonal antibody

CYP2C9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16355-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

(R) limonene 6 monooxygenase, (S) limonene 6 monooxygenase, (S) limonene 7 monooxygenase, CPC9, CYP2C, CYP2C10, CYP2C9, CYPIIC9, Cytochrome P 450MP, Cytochrome P450 2C9, Cytochrome P450 MP 4, Cytochrome P450 MP 8, Cytochrome P450 PB 1, P450IIC9, S mephenytoin 4 hydroxylase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, SMMC-7721 cells, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16355-1-AP targets CYP2C9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9566

Product name: Recombinant human CYP2C9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-162 aa of BC020754

Sequence: MDSLVVLVLCLSCLLLLSLWRQSSGRGKLPPGPTPLPVIGNILQIGIKDISKSLTNLSKVYGPVFTLYFGLKPIVVLHGYEAVKEALIDLGEEFSGRGIFPLAERANRGFGIVFSNGKKWKEIRRFSLMTLRNFGMGKRSIEDRVQEEARCLVEELRKTKGG

相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily C, polypeptide 9
Calculated molecular weight162aa,18 kDa; 490aa,56 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC020754
Gene Symbol CYP2C9
Gene ID (NCBI) 1559
RRIDAB_3085487
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP11712
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP2C9 (Cytochrome P450 Family 2 Subfamily C Member 9) is a crucial cytochrome P450 enzyme, which plays a significant role in the metabolism, by oxidation, of both xenobiotic and endogenous compounds. CYP2C9 is primarily expressed in the liver, and the expression level is reported to be the second highest among CYP isoforms(PMID: 20150829).

Protocols

Product Specific Protocols
IHC protocol for CYP2C9 antibody 16355-1-APDownload protocol
WB protocol for CYP2C9 antibody 16355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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