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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP2C8 Recombinant antibody (85717-1-RR)

    WB analysis using 85717-1-RR (same clone as 85717-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85717-1-RR (CYP2C8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85717-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CYP2C8 Recombinant antibody (85717-1-RR)

    IHC staining of mouse liver using 85717-1-RR (same clone as 85717-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85717-1-RR (CYP2C8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85717-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CYP2C8 Recombinant antibody (85717-1-RR)

    IHC staining of mouse liver using 85717-1-RR (same clone as 85717-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85717-1-RR (CYP2C8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85717-1-PBS in a different storage buffer formulation.

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CYP2C8 Recombinant antibody, PBS Only

CYP2C8 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85717-1-PBS
Clone No.242948A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CP2C8, 242948A3, CPC8, CYP2C8/9/18/19, CYPIIC8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85717-1-PBS targets CYP2C8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CYP2C8 fusion protein Ag9898 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily C, polypeptide 8
Calculated molecular weight 490 aa, 56 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC020596
Gene Symbol CYP2C8
Gene ID (NCBI) 1558
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10632
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CYP2C8(Cytochrome P450 2C8) is also named as CYPIIC8, cytochrome P450 IIC2, cytochrome P450 MP-12, cytochrome P450 MP-20, cytochrome P450 form 1 and S-mephenytoin 4-hydroxylase. It belongs to the cytochrome P450 family. It is a monooxygenase involved in an NADPH-dependent electron transport pathway and a hepatic drug-metabolizing enzymes that oxidizes therapeutic drugs such as cerivastatin and endobiotics such as retinoic acid and arachidonic acid. It has 2 isoforms produced by alternative splicing.