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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using CYP27A1 Polyclonal antibody (14739-1-AP)

    WB analysis of mouse liver using 14739-1-AP (same clone as 14739-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14739-1-AP (CYP27A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using CYP27A1 Polyclonal antibody (14739-1-AP)

    WB analysis of NIH/3T3 using 14739-1-AP (same clone as 14739-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14739-1-AP (CYP27A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP27A1 Polyclonal antibody (14739-1-AP)

    IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP27A1 Polyclonal antibody (14739-1-AP)

    IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP27A1 Polyclonal antibody (14739-1-AP)

    IHC staining of human liver using 14739-1-AP (same clone as 14739-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 14739-1-AP (CYP27A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP27A1 Polyclonal antibody (14739-1-AP)

    IHC staining of human liver using 14739-1-AP (same clone as 14739-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 14739-1-AP (CYP27A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

CYP27A1 Polyclonal antibody, PBS Only

CYP27A1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14739-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Cytochrome P450 27, Cytochrome P 450C27/25, CYP27, CTX, CP27

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14739-1-PBS targets CYP27A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6408

Product name: Recombinant human CYP27A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 183-531 aa of BC051851

Sequence: DDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAICYILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWNAIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKETLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWLRNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELKSVARIVLVPNKKVGLQFLQRQC

相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 27, subfamily A, polypeptide 1
Calculated molecular weight 60 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC051851
Gene Symbol CYP27A1
Gene ID (NCBI) 1593
RRIDAB_2089276
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02318
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and 27-hydroxylation of cholesterol in most tissues(PMID:17088262). It belongs to the cytochrome P450 family. CYP27A1 catalyses an important sterol elimination pathway in the macrophage, and consequently may protect against atherosclerosis.

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