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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP26A1 Recombinant monoclonal antibody (84699-1-RR)

    WB analysis using 84699-1-RR (same clone as 84699-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84699-1-RR (CYP26A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CYP26A1 Recombinant monoclonal antibody (84699-1-RR)

    WB analysis using 84699-1-RR (same clone as 84699-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84699-1-RR (CYP26A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CYP26A1 Recombinant monoclonal antibody (84699-1-RR)

    IHC staining of mouse skeletal muscle using 84699-1-RR (same clone as 84699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84699-1-RR (CYP26A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CYP26A1 Recombinant monoclonal antibody (84699-1-RR)

    IHC staining of mouse skeletal muscle using 84699-1-RR (same clone as 84699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84699-1-RR (CYP26A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84699-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CYP26A1 Recombinant monoclonal antibody (84699-1-RR)

    IF Staining of human placenta using 84699-1-RR (same clone as 84699-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CYP26A1 antibody (84699-1-RR, Clone: 241816D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84699-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CYP26A1 Recombinant monoclonal antibody (84699-1-RR)

    FC experiment of HepG2 using 84699-1-RR (same clone as 84699-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CYP26A1 Recombinant antibody (84699-1-RR, Clone:241816D11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84699-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84699-1-RR

    Affinity and Kinetic Characterization of 84699-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84699-1-RR against Human CYP26A1 were performed. The affinity constant is below 1 pM.

CYP26A1 Recombinant monoclonal antibody, PBS Only

CYP26A1 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 84699-1-PBS
Clone No.241816D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

241816D11, CP26, CYP26, Cytochrome P450 26A1, Cytochrome P450RAI

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84699-1-PBS targets CYP26A1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag24898

Product name: Recombinant human CYP26A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 110-220 aa of BC157063

Sequence: VHWPASVRTILGSGCLSNLHDSSHKQRKKVIMRAFSREALECYVPVITEEVGSSLEQWLSCGERGLLVYPEVKRLMFRIAMRILLGCEPQLAGDGDSEQQLVEAFEEMTRN

相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 26, subfamily A, polypeptide 1
Calculated molecular weight 497 aa, 56 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC157063
Gene Symbol CYP26A1
Gene ID (NCBI) 1592
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO43174
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CYP26A1 (Cytochrome P450 Family 26 Subfamily A Member 1), also known as Cytochrome P450 26A1, CP26, CYP26, P450RAI, and P450RAI1, is a member of the cytochrome P450 superfamily of enzymes, involved in retinoic acid (RA) metabolism and synthesis of cholesterol, steroids, and other lipids. CYP26A1 is essential for normal hindbrain patterning, vertebral identity, and development of posterior structures(PMID: 11157778).

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