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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CYP1A2-Specific Recombinant monoclonal antibody (84751-1-RR)

    WB analysis of HepG2 using 84751-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CYP1A2-Specific Recombinant monoclonal antibody (84751-1-RR)

    WB analysis of HEK-293 using 84751-1-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CYP1A2-Specific Recombinant monoclonal antibody (84751-1-RR)

    IHC staining of mouse liver using 84751-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human Hepatocellular cancer using CYP1A2-Specific Recombinant monoclonal antibody (84751-1-RR)

    IHC staining of Human Hepatocellular cancer using 84751-1-RR

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer tissue slide using 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human Hepatocellular cancer using CYP1A2-Specific Recombinant monoclonal antibody (84751-1-RR)

    IHC staining of Human Hepatocellular cancer using 84751-1-RR

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CYP1A2-Specific Recombinant monoclonal antibody (84751-1-RR)

    IHC staining of mouse liver using 84751-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CYP1A2-Specific Recombinant monoclonal antibody

CYP1A2-Specific Recombinant Antibody for WB, IHC, ELISA
Cat No. 84751-1-RR
Clone No.241922C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

CYP1A2, 241922C4, Cholesterol 25-hydroxylase, CP12, CP1A2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells
Positive IHC detected inHuman Hepatocellular cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84751-1-RR targets CYP1A2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 1, subfamily A, polypeptide 2
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_000761
Gene Symbol CYP1A2
Gene ID (NCBI) 1544
RRIDAB_3672134
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP05177
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.

Protocols

Product Specific Protocols
WB protocol for CYP1A2-Specific antibody 84751-1-RRDownload protocol
IHC protocol for CYP1A2-Specific antibody 84751-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols