Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICC

WB analysis of human placenta using 16554-1-AP (same clone as 16554-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
WB analysis using 16554-1-AP (same clone as 16554-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 16554-1-AP (same clone as 16554-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP19A1 antibody (16554-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CYP19A1 Polyclonal antibody, PBS Only

CYP19A1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 16554-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

ARO, ARO1, Aromatase, CPV1, CYAR

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16554-1-PBS targets CYP19A1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9765 Product name: Recombinant human CYP19A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC022896 Sequence: MVLEMLNPIHYNITSIVPEAMPAATMPVLLLTGLFLLVWNYEGTSSIPGPGYCMGIGPLISHGRFLWMGIGSACNYYNRVYGEFMRVWISGEETLIISKSSSMFHIMKHNHYSSRFGSKLGLQCIGMHEKGIIFNNNPELWKTTRPFFMKALSGPGLVRMVTVCAESLKTHLLLFTPASVN 相同性解析による交差性が予測される生物種
Full Name	cytochrome P450, family 19, subfamily A, polypeptide 1
Calculated molecular weight	20 kDa, 25 kDa, 58 kDa
Observed molecular weight	55-58 kDa
GenBank accession number	BC022896
Gene Symbol	CYP19A1
Gene ID (NCBI)	1588
RRID	AB_2088670
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P11511
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human placenta using 16554-1-AP (same clone as 16554-1-PBS)

WB analysis using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human gliomas using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human gliomas using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of rat brain using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of rat brain using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human skeletal muscle using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human skeletal muscle using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human kidney using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human kidney using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human testis using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human testis using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human spleen using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human lung using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human lung using 16554-1-AP (same clone as 16554-1-PBS)

IHC staining of human ovary using 16554-1-AP (same clone as 16554-1-PBS)